Increased yield, quality and productivity–HyperGRO™ makes it possible.
Nature Technology’s proprietary HyperGRO™ plasmid DNA manufacturing platform is an advanced, fed-batch, nutrient-limited process that utilizes a temperature shift to reach record high yields of up to 3.5 g/L. The HyperGRO™ platform host strains and process show significant improvement on vector yield, quality, and transgene expression. Nanoplasmid™ and RNA-OUT specific strains improve replication of DNA vectors and provide scalable high yield manufacturing using HyperGRO™. The REVIVER strain for stable production of ITR and large palindrome-containing plasmids provides stabilization and high yield manufacturing benefits for AAV ITR vectors. The HyperGRO™ platform has been utilized by multiple CMOs and pharmaceutical companies for clinical and commercial production.
Production of highly-purified research-grade plasmid DNA at NTC’s Lincoln, Nebraska, USA plant range from 1mg to multi-gram scale, and production capabilities for GMP-grade DNA at NTC’s CMO partners range from a hundred milligrams upward.
Our low metabolic-burden cell banking and fed-batch process produces higher specific and volumetric yield than typical batch and fed-batch processes: Up to 3.5 g/L plasmid yield with optimized NTC vectors.