Nature Technology’s proprietary HyperGRO™ plasmid DNA manufacturing platform is an advanced, fed-batch, nutrient-limited process that utilizes a temperature shift to reach record high yields of up to 3.5 g/L. The HyperGRO™ platform host strains and process show significant improvement on vector yield, quality, and transgene expression. Nanoplasmid™ and RNA-OUT specific strains improve replication of DNA vectors and provide scalable high yield manufacturing using HyperGRO™. The REVIVER strain for stable production of ITR and large palindrome-containing plasmids provides stabilization and high yield manufacturing benefits for AAV ITR vectors. The HyperGRO™ platform has been utilized by multiple CMOs and pharmaceutical companies for clinical and commercial production.