Nature Technology Corporation is now part of Aldevron. NTC Technologies product information is moving on December 30, 2022.
Research-grade PLASMID manufacturing
Research-Grade PLASMID Manufacturing
At scales ranging from 1 mg to multi-gram quantities.
NTC’s proprietary plasmid production technologies allow us to produce a wide variety of plasmid constructs with high quality and rapid turnaround. Research grade plasmid manufacturing services are available for Nanoplasmids™, RNA-OUT vectors, and other client plasmids.
NTC will:
Transform client plasmid into production host
Create a glycerol stock cell bank using “low metabolic burden technology”; this cell bank is maintained at NTC at no additional fee for additional future orders of the same plasmid
Grow, and purify the desired quantity of pDNA, using endotoxin-free reagents and procedures
Formulate the DNA according to client defined specification (default is 0.5mg/ml in TE buffer)
Ship the purified plasmid DNA and provide a Certificate of Analysis
Widely applicable
Production of highly-purified research-grade plasmid DNA at NTC’s Lincoln, Nebraska, USA plant range from 1mg to multi-gram scale, and production capabilities for GMP-grade DNA at NTC’s CMO partners range from a hundred milligrams upward.
- Alkaline lysis
- Anion exchange purification
- Proprietary polishing step for ultra-low endotoxin and other host cell impurity levels
Specifications:
Concentration: specified by client, typically 0.5-5 mg/mL
A260/A280: 1.8-2.0
Appearance: Clear, colorless solution
Identity: Matches client defined restriction digest information
Host gDNA: <5%
Host RNA: <1%
Endotoxin: <40 EU/mg
Download a Sample of our Large Scale C of A
Additional large scale QC testing available, please inquire (bioburden, host protein)
Standard Purity Endotoxin-Free (<100 EU/mg)
Endotoxin-free (<100 EU/mg) transfection grade plasmid suitable for a wide variety of experiments and preclinical studies
- Shake flask or batch fermentation growth
- Alkaline lysis
- Anion exchange purification
Specifications
Concentration: specified by client, typically 0.5-5 mg/mL
A260/A280: 1.8-2.0
Appearance: Clear, colorless solution
Identity: Matches client defined restriction digest information
Additional QC testing available
High Purity for Primary Cell Transfection
NTC’s High Purity Research Grade Plasmid process typically achieves <0.5 EU/mg, and was developed to produce plasmid for primary cell transfection (for example nucleofection into T-cells). Primary cells are very sensitive to trace bacterial contaminants present in standard plasmid preps and removal of these trace contaminants is recommended to avoid innate activation/toxicity for such applications.
- Shake flask or batch fermentation growth
- Alkaline lysis
- Anion exchange purification
- Proprietary polishing step for ultra-low endotoxin and other host cell impurity levels
Specifications:
Concentration: specified by client, typically 0.5-5 mg/mL
A260/A280: 1.8-2.0
Appearance: Clear, colorless solution
Identity: Matches client defined restriction digest information
Download a Sample of our Large Scale C of A
Additional small scale QC testing available, please inquire (bioburden, host gDNA, host RNA, endotoxin, host protein)